MrMik on Nostr: I bought a bunch of LC (liquid culture) syringes and inoculated 4% honey water with ...
I bought a bunch of LC (liquid culture) syringes and inoculated 4% honey water with them.
Seems to work perfectly almost every time, for more than 20 species. Not that I managed to get many to actually fruit (yet), but the LCs are alive and kicking.
I also inoculated an agar plate from a spore print and than ran it through another few agar plates, although I could not see contamination, and then sucked a bit of the agar plate mycelium into a syringe and injected it into a liquid culture jar. Perfect result first time (I built a laminar flow box of sorts for the agar plate part). Multiplying the LC into more LC jars is super easy and reliable, but you definitely need a starting culture that has no other organisms in it.
I also managed to take a tissue sample through the grow bag of a lions mane fruiting substrate kit and injected it into LC, that mother LC has been multiplied into several other LC jars and been used to inoculate fruiting substrate with good results.
Once you have a clean liquid culture, you can successfully multiply the mycelium without a laminar flow hood or still air box.
Seems to work perfectly almost every time, for more than 20 species. Not that I managed to get many to actually fruit (yet), but the LCs are alive and kicking.
I also inoculated an agar plate from a spore print and than ran it through another few agar plates, although I could not see contamination, and then sucked a bit of the agar plate mycelium into a syringe and injected it into a liquid culture jar. Perfect result first time (I built a laminar flow box of sorts for the agar plate part). Multiplying the LC into more LC jars is super easy and reliable, but you definitely need a starting culture that has no other organisms in it.
I also managed to take a tissue sample through the grow bag of a lions mane fruiting substrate kit and injected it into LC, that mother LC has been multiplied into several other LC jars and been used to inoculate fruiting substrate with good results.
Once you have a clean liquid culture, you can successfully multiply the mycelium without a laminar flow hood or still air box.